Orwa JA, Gerven AV, Roets E & Hoogmartens J
Development and validation of a liquid chromatography method for analysis of colistin sulphate.

Chromatographia, 51: 433-436, 2000
ISSN: 0009-5893 Chromatographia (PubMed)

Abstract
The peptide antibiotic Colistin has been successfully separated into more than thirty components on a YMCPack Pro, C-18 column with a mobile phase of acetonitrile - sodium sulphate (0.7 % m/v) - phosphoric acid (6.8 % v/v dilution of 85 % m/m H3PO4) - water (21.5:50:5:23.5) at 1.0 mL per min and detection at 215 nm. Baseline resolution of the major components, colistin A and B, and many minor components was obtained. Robustness was evaluated by performing a full-factorial design experiment. The method showed good selectivity, repeatability, linearity and sensitivity.